Mitochondrial Boost Reverses Protein Clumping in Aging and Alzheimer's - Neuroscience News

Buck researchers have recently completed a systematic study in worms that paints an intricate picture of the connections between insoluble proteins in neurodegenerative diseases and aging.

Furthermore, the work demonstrated an intervention that could reverse the toxic effects of the aggregates by boosting mitochondrial health.

"Based on our discoveries, targeting insoluble proteins could provide a strategy for the prevention and treatment of a variety of age-related diseases," said Edward Anderton, PhD, a postdoctoral fellow in Gordon Lithgow's lab and co-first author of a study that appears in the May 16 issue of the journal GeroScience.

"Our study shows how maintaining healthy mitochondria can combat protein clumping linked to both aging and Alzheimer's," said Manish Chamoli, PhD, a research scientist in Gordon Lithgow's and Julie Andersen's lab, and co-first author of the study.

"By boosting mitochondrial health, we can potentially slow down or reverse these harmful effects, offering new ways to treat both aging and age-related diseases."

Results support the geroscience hypothesis

The strong link between insoluble proteins promoting normal aging and diseases also builds a case for the bigger picture of how aging and age-related diseases occur. "We would argue that this work really supports the geroscience hypothesis that there is a common pathway to Alzheimer's disease and aging itself," said Buck Professor Gordon Lithgow. PhD, Vice President of Academic Affairs and the senior author of the study.

"Aging is driving the disease, but the factors that put you on the track toward the disease actually occur very early."

The fact that the team found a core insoluble proteome enriched with numerous proteins that had not been considered before creates new targets for exploration, said Lithgow. "In some ways it raises the flag about whether we should be thinking about what Alzheimer's looks like in very young people," he said.

Beyond amyloid and tau

The focus of most research on Alzheimer's disease to date has been targeting accumulations of two proteins: amyloid beta and tau. But there are actually thousands of other proteins in these insoluble aggregations, said Anderton, and their role in Alzheimer's disease was unknown.

Additionally, he added, their lab and others' have observed that during the normal disease-free aging process there is also an accumulation of insoluble proteins. These insoluble proteins from aged animals, when mixed with amyloid beta in the test tube, accelerate the aggregation of the amyloid.

What was the connection between the accumulation aggregates Alzheimer's and disease-free aging, the team wondered. Focusing on the amyloid beta protein, they used a strain of the microscopic worm Caenorhabditis elegans, long been used in aging studies, that has been engineered to produce human amyloid protein.

Anderton said the team suspected they might see that amyloid beta is driving some level of insolubility in other proteins.

"What we found is that amyloid beta causes a massive amount of insolubility, even in a very young animal," said Anderton. They found that there is a subset of proteins that seem to be very vulnerable to becoming insoluble, either by adding amyloid beta or during the normal aging process. They called that vulnerable subset the "core insoluble proteome".

The team went on to demonstrate that the core insoluble proteome is full of proteins that have already been linked to different neurodegenerative diseases in addition to Alzheimer's disease, including Parkinson's disease, Huntington's disease and prion disease.

"Our paper shows that amyloid could be acting as a driver of this normal aging aggregation," said Anderton.

"Now we've got clear evidence, I think for the first time, that both amyloid and aging are affecting the same proteins in a similar way. It's quite possibly a vicious cycle where aging is driving insolubility and amyloid beta is also driving insolubility, and they're just making each other worse."

The amyloid protein is very toxic to the worms and the team wanted to find a way to reverse that toxicity.

"Since hundreds of mitochondrial proteins become insoluble both during aging and after expressing amyloid beta, we thought if we can boost the mitochondrial protein quality using a compound, then maybe we can reverse some of the negative effects of amyloid beta," said Anderton.

That's exactly what they found, using Urolithin A, a natural gut metabolite produced when we eat raspberries, walnuts, and pomegranates which is known to improve mitochondrial function: it significantly delayed the toxic effects of amyloid beta.

"Something that was glaringly obvious from our dataset is that the importance of mitochondria keeps coming up," said Anderton. A takeaway, the authors say, is the reminder that the health of mitochondria is critical to overall health.

"Mitochondria have a strong link with aging. They've got a strong link with amyloid beta," he said. "I think ours is one of the few studies that shows that insolubility and aggregation of those proteins might be the link between the two."

"Because the mitochondria are so central to all of this, one way to break the vicious cycle of decline is to replace damaged mitochondria with new mitochondria," said Lithgow. "And how do you do that? You exercise and follow a healthy diet."

Other Buck researchers involved in the study include Dipa Bhaumik, Christina D. King, Xueshu Xie, Anna Foulger, Julie K. Andersen, and Birgit Schilling.

Funding: This work was supported in part through funds from the National Institute on Aging (NIA RF1AG057358 NIA U01AG045844), a National Institutes of Health shared instrumentation grant, and the Larry L. Hillblom Medical Foundation.

About this Alzheimer's disease, genetics, and aging research news

Author: Kris Rebillot

Source: Beck Institute

Contact: Kris Rebillot - Beck Institute

Image: The image is credited to Neuroscience News

Original Research: Open access.

"Amyloid β accelerates age-related proteome-wide protein insolubility" by Edward Anderton et al. GeroScience

Abstract

Amyloid β accelerates age-related proteome-wide protein insolubility

Loss of proteostasis is a highly conserved feature of aging across model organisms and results in the accumulation of insoluble protein aggregates.

Protein insolubility is also a unifying feature of major age-related neurodegenerative diseases, including Alzheimer's Disease (AD), in which hundreds of insoluble proteins associate with aggregated amyloid beta (Aβ) in senile plaques.

Despite the connection between aging and AD risk, therapeutic approaches to date have overlooked aging-driven generalized protein insolubility as a contributing factor. However, proteins that become insoluble during aging in model organisms are capable of accelerating Aβ aggregation in vitro and lifespan in vivo.

Here, using an unbiased proteomics approach, we questioned the relationship between Aβ and age-related protein insolubility.

Specifically, we uncovered that Aβ expression drives proteome-wide protein insolubility in C. elegans, even in young animals, and this insoluble proteome is highly similar to the insoluble proteome driven by normal aging, this vulnerable sub-proteome we term the core insoluble proteome (CIP).

We show that the CIP is enriched with proteins that modify Aβ toxicity in vivo, suggesting the possibility of a vicious feedforward cycle in the context of AD.

Importantly, using human genome-wide association studies (GWAS), we show that the CIP is replete with biological processes implicated not only in neurodegenerative diseases but also across a broad array of chronic, age-related diseases (CARDs).

This provides suggestive evidence that age-related loss of proteostasis could play a role in general CARD risk.

Finally, we show that the geroprotective, gut-derived metabolite, Urolithin A, relieves Aβ toxicity, supporting its use in clinical trials for dementia and age-related diseases.